Full-length transcriptome analysis revealed that 2,4-dichlorophenoxyacetic acid promoted in vitro bulblet initiation in lily by affecting carbohydrate metabolism and auxin signaling

Bulblet initiation, including adventitious bud initiation and bulblet formation, is a crucial process for lily other bulbous flowers that are commercially propagated by vegetative means. Here, hybrid strategy combining Pacific Biosciences (PacBio) full-length sequencing Illumina RNA (RNA-seq), high-quality transcripts of L. brownii ( Lb ) its variety, var. giganteum Lbg ), during in vitro were obtained. A total 53,576 65,050 non-redundant generated, respectively. Morphological observation showed possessed stronger capacity to generate bulblets than , 1 mg L −1 2,4-dichlorophenoxyacetic acid (2,4-D) significantly increased regeneration rate two lilies. Screening differentially expressed (DETs) between different stages Mfuzz analysis 0 DAT was the stage with most complex transcriptional change, carbohydrate metabolism pathway enriched. In addition, 6,218 8,965 DETs screened 2,4-D-treated group control 2,4-D application had evident effects on expression genes involved auxin signaling pathway, such as TIRs, ARFs, Aux/IAAs, GH3s SAURs. Then, we compared profiles treatments. SUSs, SUTs, TPSs, AGPLs, GBSSs SSs significant responses initiation. The CWINs, SUTs SWEETs upregulated starch degradation (AMYs BAMs) inhibited synthesis (AGPLs, SSs). SBEs but not . Significant co-expression signaling, together transcription factors bHLHs, MYBs, ERFs C3Hs. This study indicates coordinate regulation serving basis further studies molecular mechanism flowers.